Object Details
user
Genentech, Inc.
Description (Brief)
This silver metal canister once contained Sau 3AI, an enzyme commonly used in molecular biology. Sau 3AI belongs to a class of enzymes known as restriction enzymes, which are useful for their ability to cleave DNA only at locations containing specific sequences of nucleotides, the small chemical units which make up the longer DNA molecule. Sau 3AI recognizes the sequence GATC and will cut before the G.
This particular canister of Sau 3AI was used to create recombinant DNA molecules at Genentech, a biotechnology company, in the early 1980s.
Source:
GeneON, “Sau3AI.” http://www.taq-dna.com/sau3ai-_91.html
Location
Currently not on view
Credit Line
Gift of Genentech
date made
1985-09
ID Number
2012.0198.28
accession number
2012.0198
catalog number
2012.0198.28
Object Name
Sau 3AI
Physical Description
Solution in Tris-HCl, 20 mmol/l (overall active ingredients)
NaCl, 50 mmol/l (overall active ingredients)
EDTA, 0.1 mmol/l (overall active ingredients)
Beta-mercaptoethanol, 5 mmol/l (overall active ingredients)
glycerol, 50% (v/v) (overall active ingredients)
metal (overall material)
Sau 3AI (overall active ingredients)
Measurements
overall: 2 7/8 in x 1 1/4 in; 7.3025 cm x 3.175 cm
place made
West Germany
used
United States: California, South San Francisco
See more items in
Medicine and Science: Biological Sciences
Biotechnology and Genetics
Science & Mathematics
Data Source
National Museum of American History
Link to Original Record
Record ID
nmah_1427306